Single Step Analysis of Catalysis of Pyruvate Oxidase from Lactobacillus plantarum. Kinetics, Mechanism and Regulation
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Involvement of pyruvate oxidase activity and acetate production in the survival of Lactobacillus plantarum during the stationary phase of aerobic growth.
In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxidase activity under the tested conditions. We show here that L. plantarum has two major pyruvate ox...
متن کاملPhosphate mediates electron transfer in pyruvate oxidase from Lactobacillus plantarum
Phosphate mediates electron transfer in pyruvate oxidase from Lactobacillus plantarum /?~ J 2tfr 6 Introduction Pyruvate oxidase from Lactobacillus plantarum (LpPOX, EC 1.2.3.3) is homotetrameric flavoenzyme with a subunit molecular mass of 65.5 kDa composed 603 residues. Each subunit contains one tightly and noncovalently bound FA thiamin diphosphate (ThDP), the biologically active form of vit...
متن کاملActivation of thiamin diphosphate and FAD in the phosphatedependent pyruvate oxidase from Lactobacillus plantarum.
The phosphate- and oxygen-dependent pyruvate oxidase from Lactobacillus plantarum is a homotetrameric enzyme that binds 1 FAD and 1 thiamine diphosphate per subunit. A kinetic analysis of the partial reactions in the overall oxidative conversion of pyruvate to acetyl phosphate and CO2 shows an indirect activation of the thiamine diphosphate by FAD that is mediated by the protein moiety. The rat...
متن کاملCharacterization and functional analysis of the poxB gene, which encodes pyruvate oxidase in Lactobacillus plantarum.
The pyruvate oxidase gene (poxB) from Lactobacillus plantarum Lp80 was cloned and characterized. Northern blot and primer extension analyses revealed that transcription of poxB is monocistronic and under the control of a vegetative promoter. poxB mRNA expression was strongly induced by aeration and was repressed by glucose. Moreover, Northern blotting performed at different stages of growth sho...
متن کاملThe catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography.
Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here...
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تاریخ انتشار 2008